| 高产谷胱甘肽酵母菌株的选育 |
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| 引用本文： | 杜丽平,肖冬光,时丽萍. 高产谷胱甘肽酵母菌株的选育[J]. 酿酒科技, 2010, 0(2): 47-49 |
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| 作者姓名： | 杜丽平 肖冬光 时丽萍 |
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| 作者单位： | 工业微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300457 |
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| 摘 要： | 以BY-14面包酵母（Saccharomyces cerevisiae）为出发菌株，通过紫外线和氮离子注入复合诱变，氯化锌、乙硫氨酸耐性平板筛选，获得一株高产谷胱甘肽的面包酵母优良菌株（S．cerevisiae）BL-23。该菌株经摇瓶发酵谷胱甘肽产量为113．46mg／L，较出发菌株提高62％，每1g干细胞含谷胱甘肽20．86mg，较出发菌株提高56％。传代培养结果表明，该菌株具有稳定的遗传性能。
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| 关 键 词： | 微生物 谷胱甘肽 酿酒酵母 诱变育种 |
Breeding of Saccharomyces cerevisiae Strains with High-yield of Glutathione |
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| DU Li-ping,XIAO Dong-guang,SHI Li-ping. Breeding of Saccharomyces cerevisiae Strains with High-yield of Glutathione[J]. Liquor-making Science & Technology, 2010, 0(2): 47-49 |
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| Authors: | DU Li-ping XIAO Dong-guang SHI Li-ping |
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| Affiliation: | Key Lab of Industrial Microbiology/a>;Ministry of Education/a>;Tianjin Industrial Microbiology Key Lab/a>;Tianjin University of Science and Technology/a>;Tianjin 300457/a>;China |
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| Abstract: | Saccharomyces cerevisiae BY-14 was used as parent strain and then mutagenized by ultraviolet radiation and ion implantation,and a mutant strain named as S.cerevisiae BL-23 with high-yield of glutathione was obtained through zinc chloride and ethionine tolerance flat screening.The yield of glutathione by BL-23 strain fermentation in shake-flask was 113.46 mg/L,62 % higher than that by BY-14 strain fermentation,and the content of glutathione in per gram stem cells was 20.86 mg/g,56 % higher than that by BY-14... |
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| Keywords: | microbe glutathione Saccharomyces cerevisiae mutation breeding |
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