超高压处理诸因素对辣根过氧化物酶活力的影响

目的:研究超高压处理对辣根过氧化物酶活力的影响。方法:实验压力为0.1～500MPa,温度为20～60℃,保压时间为5～30min,酶溶液pH7.0。结果:①在常压常温条件下,在酶溶液为pH7.0时酶活力最大,为其最适酸碱度。②在处理温度为40℃、保压时间为10min和酶溶液pH7.0的条件下,压力对酶活力有显著影响;在100MPa附近的低压处理时,酶活力会反常升高;大于400MPa处理时,酶活力下降趋势缓慢。③在处理压力为500MPa、保压时间为10min、酶溶液pH7.0条件下,在40℃以下的温度范围内,酶的活力下降趋势缓慢;40℃以后,酶活力随温度升高下降迅速。④在500MPa、40℃、pH值为7.0的条件下,保压25min辣根过氧化物酶的残留活力接近最低水平,进一步延长保压时间对酶的活力影响甚微;保压时间不是影响酶活力的主要因素。结论:超高压处理对辣根过氧化物酶活力影响显著;压力、温度和保压时间对酶活力均产生较大影响。

Effects of High Pressure(HP) Treatment Parameters on Horseradish Peroxidase(HRP) Activity

Objective: The effects of high pressure(HP) treatment parameters on horseradish peroxidase(HRP) activity was studied. Method: The experiments was carried out under the high hydrostatic pressure (0.1～500 MPa), temperature (20～60 ℃), dwell time (2～34 min) and pH7.0 of the enzymatic solution. Result: ① The pH7.0 of the enzymatic solution is optimal acidity, and HRP activity value is highest at environmental pressure(0.1MPa) and ambient temperature(20℃). ② The effect of compression is significant on HRP activity under the treatment temperature 40 ℃, dwell time 10min and pH7.0; HRP activity increases abnormally in treatment of about 100MPa, and decreases slowly above 400 MPa. ③ HRP activity decreases slowly under temperature of less than 40 ℃ at the pressure of 500MPa and dwell time of 10min pH7.0; Decreases rapidly with increase of temperature above 40 ℃. ④ HRP activity arrives at the least level after compression of 25 min, increases little with prolonging further time; Dwell time is not main factor affecting HRP activity in HP treatment. Conclusion: HP treatment remarkably influences HRP activity. The pressure, temperature and dwell time is remarkable factors in HP treatment on HRP.