气相色谱法测定饼干中的抗氧化剂BHA、BHT与TBHQ

建立了气相色谱法同时测定饼干中的3种抗氧化剂丁基羟基茴香醚（BHA）、二丁基羟基甲苯（B肿）和特丁基对苯二酚（TBHQ）含量的方法。样品采用无水乙醇提取，经净化、离心后直接进样测定。试验得出方法最低检出限BHA和BHT为1．0mg／kg，TBHQ为2．0mg／kg，3种抗氧化剂加标回收率在86．70％-103．02％之间，相对偏差均小于3％，线性范围在2μg／mL-200μg／mL之间，相关系数均大于0．999。该方法简便、快速、稳定、可靠，适用于饼干中BHA、BHT和TBHQ含量的同时快速检测。

Determination of BHA,BHT, and TBHQ in biscuit by gas chromatography

Method for determination of BHA, BHT,and TBHQ in biscuit by gas chromatography was established in this paper. The sample was extracted with absolute alcohol. It was directly determined by GC after purification and centrifugation. Results showed that the detection limits of BHA,BHT,and TBHQ were 1.0 mg/kg, 1.0 mg/kg, 2.0 mg/kg, respectively.The recoveries of three antioxidants ranged from 86.70% to 103.02 and their relative standard deviations were less than 3%.The linear ranges were from 2μg/mL - 200μg/mL and their correlation coefficients were more than 0.999.This method is simple, rapid and stable. It can be used for the determination of BHA, BHT, and TBHQ in biscuit.