| Nd:YAG激光微束切割大麦染色体及染色体片段的分离 |
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| 引用本文： | 王槐,宋桂英,王兰岚,胡赞民,陈正华. Nd:YAG激光微束切割大麦染色体及染色体片段的分离[J]. 中国激光, 1998, 25(7): 657-660 |
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| 作者姓名： | 王槐 宋桂英 王兰岚 胡赞民 陈正华 |
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| 作者单位： | 中国科学院遗传研究所 |
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| 基金项目： | 中国科学院生物科学与技术研究特别支持课题 |
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| 摘 要： | 以适当参数的ＮｄＹＡＧ激光微束切割大麦７Ｈ染色体后再利用微细玻璃针挑取了７ＨＳ端部片段并放入Ｅｐｐｅｎｄｏｒｆ管中，建立了一种激光微束与玻璃针结合使用微切割、微分离植物染色体片段的方法，为今后植物染色体特定区域ＤＮＡ的微克隆技术提供了一种新的方法
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| 关 键 词： | 大麦，染色体微切割，Nd:YAG激光微束 |
| 收稿时间： | 1997-12-29 |
Microdissection of Chromosomes by a Nd:YAG Laser Microbeam and Isolation of Chromosomal Fragments in Hordeum Vulgare L. |
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| Wang Huai Song Guiying Wang Lanlan Hu Zanmin Chen Zhenghua. Microdissection of Chromosomes by a Nd:YAG Laser Microbeam and Isolation of Chromosomal Fragments in Hordeum Vulgare L.[J]. Chinese Journal of Lasers, 1998, 25(7): 657-660 |
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| Authors: | Wang Huai Song Guiying Wang Lanlan Hu Zanmin Chen Zhenghua |
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| Abstract: | A method for microdissection, isolation of chromosomal fragments using a laser beam incorporated with a microglassneedle was established. Firstly, a 7H chromosome of Hordeum Vulgare L. was dissected by the Nd:YAG laser beam of suitable parameters; then the fragment comprising a satellite was isolated and put into an eppendorf tube by a micro glassneedle fixed on a micromanipulator. This research provides a new method for the microdissection of special chromosomal regions. |
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| Keywords: | ordeum Vulgare L. chromosome microdissection Nd:YAG laser microbeam |
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