3D打印含铜钴基合金与PDLF细胞生物相容性初探

目的 在现有医用钴基合金材料中加入铜元素，使用选择性激光熔化技术（selected laser melting，SLM）制得一种新型含铜钴基合金，并检测其生物相容性。方法 以SLM技术制得的新型含铜钴基合金作为实验组，以SLM技术制得的不含铜元素的传统医用钴基合金作为对照组，将实验材料与人牙周膜成纤维细胞（periodontalliga-ment fibroblast，PDLF）进行共培养后，使用细胞增殖活力测定（CCK-8法）及实时无标记细胞分析技术（Real Time Cellular Analysis，RTCA）-细胞毒性测定、鬼笔环肽细胞染色、细胞流式实验，来检测材料对细胞增殖、凋亡、粘附的影响，从而评价新型含铜钴基合金的生物相容性。结果 与新型含铜钴基合金直接接触共培养后的PDLF细胞，其细胞相对增长率大于99%，属于零级细胞毒性。与新型含铜钴基合金间接接触共培养后的PDLF细胞，其整个生长增殖过程并未受到新型合金的影响。与新型含铜钴基合金共培养1天后，所有分组PDLF细胞凋亡率均小于1%，共培养3天后，所有分组细胞凋亡率均小于5%，实验组与对照组无显著性差异，且整体略低于对照组。鬼笔环肽染色后观察到细胞粘附正常，实验组与对照组之间无明显差异。结论 经SLM技术制作的含铜钴基合金在保证拥有良好抗菌性能的同时，不会对细胞产生细胞毒性，即具有良好的细胞相容性。

Biocompatibility between Cu-bearing CoCrW Alloy by 3D Printing and PDLF

The work aims to add Cu in existing medical CoCrW alloy to prepare a new Cu-bearing CoCrW alloy by selected laser melting (SLM) and test the biocompatibility. The new Cu-bearing CoCrW alloy prepared by SLM was used as the experi-mental group and the Cu-free CoCrW alloy prepared by SLM was regarded as the control group. After the experimental materials and periodontalliga-ment fibroblast were co-cultured, CCK-8 and Real Time Cellular Analysis (RTCA, cytotoxicity assay, Phalloidin cell staining and flow cytometry) were adopted to test the effects of materials on proliferation, apoptosis and adhesion of cells so as to evaluate the biocompatibility of new Cu-bearing CoCrW alloy. The RGR of PDLF cells directly contacting the new Cu-bearing CoCrW alloys after co-cultured was more than 99% and the cells were classified as zero-grade cytotoxicity. The growth of PDLF cells indirectly contacting the new Cu-bearing CoCrW alloys after co-cultured was not affected by the new alloy. The apoptosis rate of PDLF was less than 1% after co-cultured with CoCrW-Cu alloy for 1 day and less than 5% after 3 days of co-culture. There was no significant difference between the experimental group and the control group and the apoptosis rate of experimental group was lower than that of control group. Through the Phalloidin cell staining, the cell adhesion was normal and there was no significant difference between the experimental group and the control group. The Cu-bearing CoCrW-Cu alloy fabricated by SLM technology has good antibacterial performance and no cytotoxicity to cells, that is, good cell compatibility.