Selection of a reference sequence for the determination of nucleotide sequences in DNA molecules |
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Authors: | S. S. Golubev S. A. Kononogov N. V. Ravin K. G. Skryabin |
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Affiliation: | 1. All-Russia Research Institute of Metrological Service (VNIIMS), Moscow, Russia 2. Bioengineering Center, Russian Academy of Sciences, Moscow, Russia
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Abstract: | A fragment of the DNA sequence of the pUC18 plasmid is proposed as a reference sequence for the calibration and metrological assurance of genome analyzers. This fragment consists of 271 nucleotide pairs and is obtained by a reference method. This sequence is optimal for determining the metrological characteristics of genome analyzers in light of its stability and the existence of several segments with repeating nucleotides. An example is given for the calculation of a genome analyzer characteristic, namely, the probability of error in measurement of the genome sequence. |
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