| 免疫抑制酸性蛋白的分离纯化及鉴定 |
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| 引用本文： | 张为远 滕红. 免疫抑制酸性蛋白的分离纯化及鉴定[J]. 中国生物制品学杂志, 1997, 10(4): 208-210 |
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| 作者姓名： | 张为远 滕红 |
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| 作者单位： | 白求恩医料大学第二临床学院!长春，130041，白求恩医料大学第二临床学院!长春，130041 |
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| 摘 要： | 采用卵巢癌腹水为原料，经盐析、DEAE－52、SephadexG100、等电聚焦电泳，Sepharose4B反相免疫亲和层析技术，分离纯化出免疫抑制酸性蛋白（IAP）纯品，经8．0％聚丙烯酰胶凝胶电泳、免疫电泳和双向免疫交叉试验，证实其为单一成分；用此抗原免疫家兔，获得了IAP抗血清，其效价为1：32。
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| 关 键 词： | 免疫抑制酸性蛋白 层析 免疫血清 |
Separation,Purification and Characterization of Immunosuppressant Acid Protein |
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| Zhang Weiyuan, Teng Hong. Separation,Purification and Characterization of Immunosuppressant Acid Protein[J]. Chinese Journal of Bilogicals, 1997, 10(4): 208-210 |
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| Authors: | Zhang Weiyuan Teng Hong |
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| Abstract: | 篒mmunosuppressant acid protein was separated and purified from the ascites of patients with oophoroma by salting out method, DEAE-52 and Sephadex G100 column chromatographys,isoe1ectrofocusing electrophoresis and Sepharose 4B reverse immune affinity chromatography. The results of 8.0% polyacrylamide gel, electrophoresis, immunoelectrophoresis and double crossed immunoelectrophoresis proved that the purified IAP was a single component. Anti-IAP serum with a titer of 1:32 was obtained by immunizing rabbits with IAP. |
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| Keywords: | Immunosuppressant acid protein Chromatography Immune serum |
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