化学发光免疫分析技术及其在食品安全检测中的研究进展

近年来, 化学发光免疫分析因其具有特异性强、灵敏度高、线性范围宽、仪器简单、操作方便、无放射性污染等优点越来越受到人们的关注。本文简要介绍了化学发光免疫分析的类型及其原理, 根据化学发光体系中标记物质的不同以及检测体系的差别, 对化学发光免疫分析技术进行了系统的分类, 并且对各种方法进行了详细的介绍, 对其优点和不足做了透彻的分析。对该分析方法在食品安全检测中的运用, 包括检测食品中微生物、毒素、农药残留、兽药残留等进行了综述, 并展望其发展方向。

Research progress on chemiluminescence immunoassay technique and its application in food safety detection

Chemiluminescence immunoassay (CLIA) received more and more attention in recent years for its high sensitivity, wide linear range, simplicity, feasibility and non-isotopic contamination. The classification and principle of chemiluminescence immunoassay were introduced briefly in this article, and the CLIAs were classified systematically according to different marker substances of the chemiluminescence system and the difference of detection systems. A variety of methods were also introduced in detail. Moreover, its advantages and disadvantages were analyzed and the application of CLIA in food safety detection, including the detection of microorganism, toxins, pesticide residues, animal drug residues and the genetically modified food were dis-cussed. The future development of CLIA was also prospected.