Proliferation of osteoblast cells on nanotubes |
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Authors: | F Watari T Akasaka Xiaoming Li M Uo and A Yokoyama |
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Affiliation: | (1) Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan |
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Abstract: | Carbon nanotubes (CNT) have a unique structure and feature. In the present study, cell proliferation was performed on the
scaffolds of single-walled CNTs (SWCNT), multiwalled CNTs (MWCNT), and on graphite, one of the representative isomorphs of
pure carbon, for the sake of comparison. Scanning electron microscopy observation of the growth of osteoblast-like cells (Saos2)
cultured on CNTs showed the morphology fully developed for the whole direction, which is different from that extended to one
direction on the usual scaffold. Numerous filopodia were grown from cell edge, extended far long and combined with the CNT
meshwork. CNTs showed the affinity for collagen and proteins. Proliferated cell numbers are largest on SWCNTs, followed by
MWCNTs, and are very low on graphite. This is in good agreement with the sequence in the results of the adsorbed amount of
proteins and expression of alkaline phosphatase activity for these scaffolds. The adsorption of proteins would be one of the
most influential factors to make a contrast difference in cell attachment and proliferation between graphite and CNTs, both
of which are isomorphs of carbon and composed of similar graphene sheet crystal structure. In addition, the nanosize meshwork
structure with large porosity is another property responsible for the excellent cell adhesion and growth on CNTs. CNTs could
be the favorable materials for biomedical applications. |
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Keywords: | carbon nanotube scaffold osteoblast regeneration nanomaterial |
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