| 响应面方法分析代谢产物对类人胶原蛋白表达的影响 |
| |
| 引用本文： | 郭佳庆,骆艳娥,范代娣,高鹏飞,马晓轩,朱晨辉.响应面方法分析代谢产物对类人胶原蛋白表达的影响[J].中国化学工程学报,2010,18(5):830-836. |
| |
| 作者姓名： | 郭佳庆 骆艳娥 范代娣 高鹏飞 马晓轩 朱晨辉 |
| |
| 作者单位： | 1. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China |
| |
| 基金项目： | the National Natural Science Foundation of China;the Natural Science Foundation of Shaanxi Province;the Specialized Research Fund for the Doctoral Program of Higher Education of China;Xi'an Research and Development Program;Shaanxi Key Subject Program，China;the Scientific Research Program of Shaanxi Provincial Department of Education,China;the National High Technology Research and Development Program of China |
| |
| 摘 要： | Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol∙L1 glucose and 0.019 mol∙L1 nitrogen (carbon-nitrogen ratio, 4.47︰1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol∙L1 and 0.017 mol∙L1, respectively (carbon-nitrogen ratio, 3.82︰1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose in-creased.
|
| 关 键 词： | carbon-nitrogen ratio human-like collagen organic acid metabolism recombinant Escherichia coli response surface methodology |
| 收稿时间： | 2010-2-24 |
| 修稿时间： | 2010-2-24
|
Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II |
| |
| GUO Jiaqing,LUO Yan'e,FAN Daidi,GAO Pengfei,MA Xiaoxuan,ZHU Chenhui.Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II[J].Chinese Journal of Chemical Engineering,2010,18(5):830-836. |
| |
| Authors: | GUO Jiaqing LUO Yan'e FAN Daidi GAO Pengfei MA Xiaoxuan ZHU Chenhui |
| |
| Affiliation: | 1. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;2. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China |
| |
| Abstract: | Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L-1 glucose and 0.019 mol·L-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L-1 and 0.017 mol·L-1, respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased. |
| |
| Keywords: | carbon-nitrogen ratio human-like collagen organic acid metabolism recombinant Escherichia coli response surface methodology |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载全文 |
|
