| 荧光法研究Gemini表面活性剂与BSA的相互作用 |
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| 引用本文： | 刘磊,陈凌宇,郑星,吴康,冯亚珍. 荧光法研究Gemini表面活性剂与BSA的相互作用[J]. 胶体与聚合物, 2011, 29(1): 31-33. DOI: 10.3969/j.issn.1009-1815.2011.01.010 |
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| 作者姓名： | 刘磊 陈凌宇 郑星 吴康 冯亚珍 |
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| 作者单位： | 湖北大学化学化工学院,武汉,430062 |
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| 摘 要： | 应用荧光法研究了Gemini表面活性剂(G14-3-14)与牛血清白蛋(BSA)在不同温度条件下的荧光猝灭现象,利用荧光猝灭双倒数图计算了G14-3-14与BSA之间的结合常数,并根据热力学参数确定了二者之间的作用力类型.用内源荧光法求得它们在30℃和35℃温度下的结合常数分别为K1=1.01×105 L·mol-1和...
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| 关 键 词： | Gemini表面活性剂 牛血清白蛋白 相互作用 荧光光谱法 |
Spectroscopic Investigation of the Interaction Between Gemini Surfactant and Bovine Serum Albumin |
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| LIU Lei,CHEN Lingyu,ZHENG Xing,WU Kang,FENG Yazhen. Spectroscopic Investigation of the Interaction Between Gemini Surfactant and Bovine Serum Albumin[J]. Chinese Journal of Colloid and Polymer, 2011, 29(1): 31-33. DOI: 10.3969/j.issn.1009-1815.2011.01.010 |
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| Authors: | LIU Lei CHEN Lingyu ZHENG Xing WU Kang FENG Yazhen |
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| Affiliation: | LIU Lei,CHEN Lingyu,ZHENG Xing,WU Kang,FENG Yazhen(College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062) |
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| Abstract: | In this paper,a fluorescence method was used to study at different temperature the fluorescence quenching of bovine serum albumin(BSA) by its interaction with Gemini 14-3-14(G14-3-14).The analysis of fluorescence data indicated the presence of dynamic quenching mechanism in the binding.The interaction association constants of BSA and G14-3-14 were determined from a double reciprocal line Weaver-Burk plot.Fluorescence quenching constants between G14-3-14 and BSA were obtained at 303K and 308K.Fluorescence da... |
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| Keywords: | Gemini Surfactant Bovine Serum Albumin(BSA) Interaction Fluorescence Spectroscopic |
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