| 双向薄层层析测定红曲中Monacolin K |
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| 引用本文： | 胡晓清,袁梦仙,陈福生,王汝毅,周有祥. 双向薄层层析测定红曲中Monacolin K[J]. 中国酿造, 2004, 0(2): 30-33 |
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| 作者姓名： | 胡晓清 袁梦仙 陈福生 王汝毅 周有祥 |
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| 作者单位： | 1. 华中农业大学食品科技学院,武汉,430070
2. 江西农业大学国土学院,南昌,330045 |
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| 摘 要： | 采用双向薄层层析法，对红曲产品中Monacolin K的提取方法以及第1展开剂和第2展开剂分别进行了研究。结果表明，采用乙酸乙酯为提取剂，以正己烷：乙酸乙酯：无水乙醚：甲酸(20：1：5：0．5)为第1展开剂，以正己烷：氯仿：无水乙醚(30：1：5)为第2展开剂，红曲产品提取液的：Monacolin K能很好地分离和检测。在此基础上对22个红曲样品进行了分析，结果显示，其中12个样品均高低不一地含Monacolin K。
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| 关 键 词： | 红曲 红曲霉 Monacolin K 双向薄层层析 |
| 文章编号： | 0254-5071(2004)02-0030-04 |
| 修稿时间： | 2003-01-21 |
Determination of Monacolin K in Red Kojic by Two Dimensional Thin Layer Chromatograph |
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| HU Xiao-qing ,YUAN Meng-xian ,CHEN Fu-sheng ,WANG Ru-yi ,ZHOU You-xiang. Determination of Monacolin K in Red Kojic by Two Dimensional Thin Layer Chromatograph[J]. China Brewing, 2004, 0(2): 30-33 |
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| Authors: | HU Xiao-qing YUAN Meng-xian CHEN Fu-sheng WANG Ru-yi ZHOU You-xiang |
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| Affiliation: | HU Xiao-qing 1,YUAN Meng-xian 2,CHEN Fu-sheng 1,WANG Ru-yi 1,ZHOU You-xiang 1 |
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| Abstract: | The extracting method of Monacolin K in red koji and the first and the second developing solvents in two dimensional thin layer chromatograph are studied in this paper, respectively. The result shows that this kind of method, which used the ethyl acetate as the extraction reagent and n-hexane: ethyl acetate: anhydrous ether: formic acid (20:1:5:0.5) as the first developing solvent and n-hexane: chloroform: anhydrous ether (30:1:5) as the second developing solvent, can get the perfect isolation and determination to the Monacolin K in red koji. 22 red koji samples were analyzed based on the determination method. The result shows that 12 samples contained different amount of Monacolin K. |
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| Keywords: | red kojic Monascus spp. Monacolin K two dimensional thin layer chromatograph |
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