An improved portable biosensing system based on enzymatic chemiluminescence and magnetic immunoassay for biological compound detection |
| |
| Affiliation: | 1. School of Control Science and Engineering, Shandong University, Jinan 250061, China;2. School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan 250353, China;3. Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;1. Department of Medicine, University of Illinois at Chicago, Chicago, IL, United States;2. Jesse Brown VA Medical Center, Chicago, IL, United States;3. Department of Urology, University of Illinois at Chicago, Chicago, IL, United States;1. Anesthesia and Surgical ICU Department, Faculty of medicine, Beni Suef University, Egypt;2. Anesthesia, Pain and Surgical ICU Department, Faculty of medicine, Cairo University, Egypt;1. Beijing Institute of Radiation Medicine, Beijing 100850, PR China;2. Institute of Transfusion Medicine, Beijing 100850, PR China;3. College of Life Sciences & Bio-Engineering, Beijing University of Technology, Beijing 100124, PR China;4. College of Mechatronics and Automation, National University of Defense Technology, Changsha 410073, PR China;1. School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo, 255049, PR China;2. Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China;1. Department of Clinical Laboratory, Hospital Affiliated to Hubei University of Arts and Science, Jingzhou Street 39, Xiangyang 441021, PR China;2. Department of Cardiology, Hospital Affiliated to Hubei University of Arts and Science, Jingzhou Street 39, Xiangyang 441021, PR China |
| |
| Abstract: | In this paper, an improved portable biosensing system has been developed for biological compounds detection. The device is based on enzymatic chemiluminescence and magnetic immunoassay with a miniaturized flow injection module. The immunoassay procedure involved a sandwich format, in which the antigen in the sample was first captured by the immobilized primary antibody on the surface of the magnetic beads, and then recognized by the second antibody labeled with enzyme. 3-(2′-spiroadamantyl)-4-methoxy- 4-(3″-phosphoryloxy)-phenyl-1,2-dioxetane (AMPPD) as the substrate amplified the enzymatic signal for the biological compound measurement. The portable biosensing system was characterized in a series of experiments with cardiac troponin I (cTnI), a golden standard protein marker for diagnosis of myocardial infarction, as a biological substance measurement model. It provided a linear response range of cTnI from 0.1 to 50 ng mL−1 (R = 0.99). The coefficient of variation was 6.7% in the repeatability test (n = 6). The novel system has great potential applications in the rural and community hospitals and can be easily extended to a variety of protein compounds analysis. |
| |
| Keywords: | Biosensing system Enzymatic chemiluminescence Magnetic immunoassay Cardiac troponin I |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
