| 农用抗生素产生菌Streptomyce SN03菌株的诱变选育 |
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| 引用本文： | 何淼,吴元华,赵刚,赵秀香.农用抗生素产生菌Streptomyce SN03菌株的诱变选育[J].农药,2007,46(8):571-573. |
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| 作者姓名： | 何淼 吴元华 赵刚 赵秀香 |
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| 作者单位： | 沈阳农业大学 植物保护学院,沈阳,110161;辽宁省林业科学研究院,沈阳,110032 |
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| 基金项目： | 国家烟草专卖局资助项目;辽宁省科技攻关计划 |
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| 摘 要： | 以链霉菌SN03菌株为出发菌株,采用紫外线诱变结合微波诱变的处理方法,通过对致死率、正负突变率和活性最大提高率的测定,确定了紫外线和微波诱变的最适时间,结合对诱变菌株的传代稳定性测定,获得了高效菌株UV-1-W3,其发酵液的抑菌圈直径达到33.2mm,抗菌活性比出发菌株提高了30.20%。
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| 关 键 词： | Streptomyce SN03菌株 诱变 选育 紫外线 微波 |
| 文章编号： | 1006-0413（2007）08-0571-03 |
| 修稿时间： | 2006-12-30 |
Mutation Breeding of Streptomyce SN03 Strain Producing Agricultural Antibiotic |
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| HE Miao,WU Yuan-hua,ZHAO Gang,ZHAO Xiu-xiang.Mutation Breeding of Streptomyce SN03 Strain Producing Agricultural Antibiotic[J].Pesticides,2007,46(8):571-573. |
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| Authors: | HE Miao WU Yuan-hua ZHAO Gang ZHAO Xiu-xiang |
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| Affiliation: | 1 .College of Plant Protection, Shenyang Agricultural University, Shenyang 11016 1, China; 2.Liaoning Academy of Forestry Science, Shenyang 110032, China |
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| Abstract: | As a original strain,SN03 was mutated by UV and microwave. Based on the lethal rate,the positive rate,the negative rate and the increase rate of activity,the best optional time of UV and microwave irradiation were determined. Combining with the genetic stability of strains selected,the high efficient strain named UV-1-W3 was obtained. The result of antifungal activity showed that the diameter of inhibition reached up to 33.2 mm,and the activity increased by 30.20%. |
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| Keywords: | Streptomyce SN03 strain mutation breeding ultraviolet (UV) microwave |
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