| 抗幽门螺杆菌卵黄抗体的制备及其效价的评定 |
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| 引用本文： | 聂作明,杨严俊,徐榕榕,盛剑俊.抗幽门螺杆菌卵黄抗体的制备及其效价的评定[J].食品科学,2005,26(5):232-237. |
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| 作者姓名： | 聂作明 杨严俊 徐榕榕 盛剑俊 |
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| 作者单位： | 江南大学食品学院生物技术组,江苏,无锡,214036 |
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| 摘 要： | 本论文研究了抗幽门螺杆菌蛋黄抗体(IgY—HP)的制备方法。以HP超声粉碎上清和HP尿素酶混合抗原物免疫鸡蛋，从蛋黄中提取纯化得到了很高纯度的IgY—HP。ELISA实验表明，IgY—HP的抗HP效价和一般IgY相比提高了32倍。其可以应用于食品保健或各种HP相关胃病的抗菌免疫治疗中。
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| 关 键 词： | 蛋黄抗体 幽门螺杆菌 ELISA 效价 |
| 文章编号： | 1002-6630(2005)05-0232-06 |
Study on Preparation of Yolk Antibody Specific Against Helicobacter pylori and It's of Efficacy Evaluation |
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| NIE Zuo-ming,YANG Yan-jun,Xu Rong-rong,SHENG Jian-jun.Study on Preparation of Yolk Antibody Specific Against Helicobacter pylori and It''''s of Efficacy Evaluation[J].Food Science,2005,26(5):232-237. |
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| Authors: | NIE Zuo-ming YANG Yan-jun Xu Rong-rong SHENG Jian-jun |
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| Abstract: | In this paper, highly purified immunoglobulin Y against Helicobacter pylori (IgY-HP) was prepared from the egg yolk while immunized with compound antigen of supernatant from HP pulverized by ultrasonic wave while urease was purified form HP.ELISA test indicated that the efficacy for IgY-Hp was 32 times higher than that for IgY in egg yolk from nonimmunized hens. It could be applied to healthcare and to the antibacterial immune treatment for gastric disease associated with HP. |
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| Keywords: | yolk antibody Helicobacter pylori ELISA titer |
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