酶活性变化在萝卜抗TuMV分析鉴定中的应用

不同萝卜品种接种抗感芜菁花叶病毒 (TuMV )后 ,测定不同时期叶片中的过氧化物酶(POD)、多酚氧化酶 (PPO)、苯丙氨酸裂解酶 (PAL)的活性 .结果表明 ,抗病品种POD活性的略低于感病品种 ,接种后POD活性升高 ;抗病品种PPO活性比感病品种高 ,接种后抗病品种PPO活性迅速升高 ,与抗性呈正相关 ,可以作为抗性大小的衡量指标 ;抗病品种PAL活性比感病品种高 ,接种后变化无规律 .

Analysis and Identification of Radish Resistance to TuMV According to the Changes of Enzymes Activity

The activity of three enzymes in radish leaves resistant or susceptible to TuMV after inoculation was measured. The result showed that POD activity of varieties resistant to TuMV is lower than varieties susceptible to TuMV and increases after inoculation. PPO activity of varieties resistant to TuMV is higher than varieties susceptible to TuMV. PPO activity in leaves is positively related to the resistant to TuMV and can be used to scale the TuMV resistance. PAL activity of varieties resistant to TuMV is higher than varieties susceptible to TuMV. The changes of PAL have not pattern.