Cholesteryl ester metabolism in tissue culture cells: II. Source of accumulated esterified cholesterol in Fu5AH rat hepatoma cells |
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Authors: | G H Rothblat L Arbogast D Kritchevsky M Naftulin |
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Affiliation: | (1) The Wistar Institute, 36th and Spruce Sts., 19104 Philadelphia, Pennsylvania |
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Abstract: | Previous investigations had demonstrated that Fu5AH rat hepatoma cells accumulated large quantities of esterified cholesterol
when grown in hyperlipemic rabbit serum. The present investigation has determined the sources of the cellular esterified cholesterol
when the cells were grown in hyperlipemic serum. Cellular esterification of endogenous and exogenous free cholesterol contributed
10% and 30%, respectively. The remaining 60% of the accumulated cellular esterified cholesterol was derived from exogenous
(serum) cholesteryl esters. Evidence for the hydrolysis of a portion of the incorporated esterified cholesterol is presented.
A stimulation of free cholesterol incorporation and cellular esterification is elicited by hyperlipemic serum and serum lipoproteins
when compared to normolipemic serum present at equivalent exogenous cholesterol concentrations. The effect of hyperlipemic
serum is reduced by Tween-80 and Triton WR-1339. Comparative data on esterified cholesterol accumulation, free cholesterol
incorporation, and cellular cholesterol esterification in Fu5-5 rat hepatoma cells, L-cells, and rabbit aortic medial cells
are presented.
This work was done during the tenure as Established Investigator of the American Heart Association. |
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