A combinatorial approach to chemical modification of subtilisin Bacillus lentus |
| |
| Authors: | E Plettner K Khumtaveeporn X Shang JB Jones |
| |
| Affiliation: | Department of Chemistry, University of Toronto, Ontario, Canada. |
| |
| Abstract: | The reaction between methanethiosulfonate reagents and cysteine mutants of subtilisin is quantitative and can be used to prepare chemically modified mutant enzymes (CMMs) with novel properties. The virtually unrestricted structural variations possible for CMMs presents a preparative and screening challenge. To address this, a rapid combinatorial method for preparing and screening the activities of CMMs has been developed. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
