New method for fabricating an α‐fetoprotein affinity monolithic polymer array |
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| Authors: | Li Li Li‐Ru Xia He‐Ye Wang Xiao‐Dong Bi |
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| Affiliation: | 1. Institute of Facilities and Equipment in Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China;2. Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China |
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| Abstract: | Bisphenol A based epoxy acrylate (BABEA), a commercial UV‐curable material, was introduced as a crosslinker for the fabrication of an epoxy‐functionalized monolithic polymer array through UV‐initiated copolymerization with glycidyl methacrylate as the functional monomer and poly(ethylene glycol) 200 as the porogen. Scanning electron microscopy images showed that the monolithic poly(bisphenol A based epoxy acrylate‐co‐glycidyl methacrylate) [poly(BABEA‐co‐GMA)] exhibited a well‐controlled skeletal and well‐distributed porous structure. The α‐fetoprotein (AFP) immunoaffinity monolithic polymer array prepared by the immobilization of AFP on epoxy‐functionalized monolithic arrays was used as an immunosensor for chemiluminescent AFP detection. X‐ray photoelectron spectroscopy results indicate that the AFP antibody was successfully immobilized on the monolithic poly(BABEA‐co‐GMA) array. With a noncompetitive immune‐response format, the proposed AFP immunoaffinity array was demonstrated as a low‐cost, flexible, homogeneous, and stable array for AFP detection. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41792. |
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| Keywords: | photopolymerization porous materials sensors and actuators |
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