| 固定化辣根过氧化物酶生物传感器测定火腿肠中亚硝酸盐 |
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| 引用本文： | 展海军,马超越,白静.固定化辣根过氧化物酶生物传感器测定火腿肠中亚硝酸盐[J].食品研究与开发,2011,32(2):123-125. |
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| 作者姓名： | 展海军 马超越 白静 |
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| 作者单位： | 河南工业大学,化学化工学院,河南,郑州,450001 |
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| 摘 要： | 制备以电子媒介体聚苯胺和纳米TiO2固定辣根过氧化物酶(HRP)的生物传感器,研究该传感器的电化学性质。结果表明,该传感器对H2O2和NaNO2都具有很好的电催化还原性。在含有0.01mg/L～100mol/L的NaNO2(pH=9)的磷酸盐缓冲溶液中,具有良好的线性相关性,其相关系数为0.994 6,检出限为0.001 mg/L。应用于火腿肠中亚硝酸盐的测定,回收率为94%~103%。
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| 关 键 词： | 辣根过氧化物酶 亚硝酸盐 纳米二氧化钛 |
Determination of Nitrite in Ham Saysauge by Immobilized Horseradish Peroxidase Biosensor |
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| ZHAN Hai-jun,MA Chao-yue,BAI Jing.Determination of Nitrite in Ham Saysauge by Immobilized Horseradish Peroxidase Biosensor[J].Food Research and Developent,2011,32(2):123-125. |
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| Authors: | ZHAN Hai-jun MA Chao-yue BAI Jing |
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| Affiliation: | ZHAN Hai-jun,MA Chao-yue,BAI Jing(School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450001,Henan,China) |
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| Abstract: | The hydrogen peroxide biosensor has been prepared by immobilizing fixed horseradish peroxidase(HRP),with polyaniline mediation and nano-TiO2.The electrochemical performance of biosensor was studied.The results show that H2O2 and NaNO2 have good catalytic reductive.The biosensor had a good linear relationship to NaNO2(pH=9) of phosphate buffer solution in the range 0.01 mg/L-100 mol/L,the correlation coefficient was 0.994 6,and the detection limit was 0.001 mg/L.The biosensor was applied to determination of ... |
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| Keywords: | horseradish peroxidase nitrite nanometer titanium dioxide |
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