氟喹诺酮类化合物的电喷雾离子阱质谱分析

采用电喷雾离子阱质谱法在正离子检测方式下，以注射泵直接进样的方式对6种氟喹诺酮类化合物的质谱裂解行为进行碰撞诱导解离研究。获得了各化合物的多级质谱信息，通过比较各化合物结构和质谱裂解途径的异同点，发现氟喹诺酮类化合物在正离子检测方式下可生成脱CO₂、HF和CO的碎片，分别与结构中的羧基、氟原子取代基和4位羰基结构片断相对应，如果结构中7位取代基为哌嗪环，还可以生成脱CH₂＝CH-NH₂的碎片。这些特征将有助于氟喹诺酮类化合物的结构分析。

Analysis of Fluroquinolones by Electrospray Ion Trap Mass Spectrometry

To investigate the fragmentation mechanism of fluroquinolones, six compounds of fluroquinolones were analyzed using electrospray ion trap mass spectrometry by collision induced dissociation in a multi-stage MS full scan mode. The samples were infused into the ion source with a syringe pump and the mass spectrometry was operated in the positive mode. The mass spectra and structures of the six fluroquinolones were compared with each other and it was observed that fluroquinolones gave characteristic fragment ions by the neutral loss of COz, HF and CO, corresponding to the carboxy, fluorine and 4-carbonyl group in their structures. There would be another fragment ions by the elimination of CH2 =CH -NH2 if the structure was with a 7-piperazidine substituent. These characteristics can be used for future structure elucidation in studies of fluroquinolones and analogue compounds.