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Influence of different cleaning and sanitisation procedures on the removal of adhered Bacillus cereus spores
Affiliation:1. Department of Energy Resources Engineering, Seoul National University, Seoul 151-744, Republic of Korea;2. School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0373, USA;3. Oak Ridge National Laboratory, Oak Ridge, TN 37831-6181, USA;1. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna – Headquarters, Via A. Bianchi, 9, 25124 Brescia, Italy;2. Food and Drug Department, University of Parma, Sede Via del Taglio 10, 43126 Parma, Italy;3. Dipartimento di Scienze Veterinarie, University of Turin, Largo P. Braccini 2, 10095, Grugliasco, TO, Italy;4. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna – Lodi Chapter, Via Albert Einstein, 26900 Lodi, Italy
Abstract:The effectiveness of nine different sanitation procedures for the removal and control of adhered Bacillus cereus spores isolated from the dairy industry was evaluated. Four sets of experiments were produced by varying the conditioning matrix (water and UHT whole milk) and the inoculation medium of the pool of B. cereus spores (water and UHT whole milk), resulting in Ewater/water, Ewater/milk, Emilk/water, and Emilk/milk. The experiments were repeated three times and evaluated by ANOVA and Tukey's test. Sanitisation with sodium hypochlorite was more effective than peracetic acid for removal of adhesion, especially when associated with a cleaning procedure, with counts below the limit of detection (<0.7 log cfu cm?2). The experiments formed when the pool of spores was inoculated on water (Ewater/water and Emilk/water) presented greater resistance against the sanitation procedures studied. These results highlight the importance of the water quality used in the industries for sanitation of equipment.
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