hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression |
| |
| Authors: | Chuang Li Fang Qin Wei Wang Yifan Ni Mingyu Gao Mingxiong Guo Guihong Sun |
| |
| Affiliation: | 1.School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China;2.Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China; (F.Q.); (W.W.); (Y.N.); (M.G.);3.Hubei Provincial Key Laboratory of Allergy and Immunology, Wuhan 430071, China |
| |
| Abstract: | Extracellular vesicles (EVs) released by tumor cells play important roles on the remodeling of the tumor–stromal environment and on promoting tumor metastasis. Our earlier studies revealed that miR-122-5p, a type of small non-coding RNA, was dysregulated in non-small cell lung cancer (NSCLC) cell-derived EVs. In this study, we found that miR-122-5p was selectively sorted and secreted into lung cancer EVs through binding to RNA-binding protein hnRNPA2B1. In addition, we found that hnRNPA2B1 interacted with miR-122-5p through the EXO-motif. The delivering of lung cancer EVs-miR-122-5p promoted the migration of liver cells, which may play roles in establishing a pre-metastatic micro-environment and hepatic metastasis of lung cancer. Importantly, our findings revealed the molecular mechanism that RNA-binding protein controls the selective sorting of tumor-derived EV miR-122-5p, which potentially promotes lung cancer progression. |
| |
| Keywords: | extracellular vesicles miR-122-5p non-small cell lung cancer hnRNPA2B1 RNA-binding protein |
|
|
