| 高效液相色谱-荧光检测器测定水产品中孔雀石绿残留总量的研究 |
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| 引用本文： | 王骏,李建业. 高效液相色谱-荧光检测器测定水产品中孔雀石绿残留总量的研究[J]. 食品科学, 2007, 28(7): 364-367 |
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| 作者姓名： | 王骏 李建业 |
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| 作者单位： | 山东省产品质量监督检验研究院； 山东省产品质量监督检验研究院 山东济南250100； 山东济南250100； |
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| 摘 要： | 建立了水产品中孔雀石绿残留总量的液相色谱检测方法。样品中的孔雀石绿全部还原为无色孔雀石绿后,用乙腈-缓冲液提取,经净化、浓缩后,以Symmetry shieldTM RP18为固定相,以乙腈-水为流动相,使用荧光检测器检测无色孔雀石绿。无色孔雀石绿线性范围为1～160ng/ml;方法检出限为1.0μg/kg(以孔雀石绿总量计);回收率为82.6%～92.6%。
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| 关 键 词： | 高效液相色谱 荧光 水产品 孔雀石绿 |
| 文章编号： | 1002-6630（2007）07-0364-04 |
| 修稿时间： | 2006-07-01 |
Determination of Malachite Green in Aquatic Products by HPLC- FLD |
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| WANG Jun,LI Jian-ye. Determination of Malachite Green in Aquatic Products by HPLC- FLD[J]. Food Science, 2007, 28(7): 364-367 |
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| Authors: | WANG Jun LI Jian-ye |
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| Affiliation: | Shandong Institute of Supervision and Inspection on Product Quality, Jinan 250100, China |
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| Abstract: | In this paper, a method for determining the total residue of malachite green in aquatic products was developed by high- performance liquid chromatography (HPLC) with fluorescence detection. Malachite green in sample was reduced to leucomalachite green. Being extracted by acetonitrile-buffer mixture, cleaned-up and condensed, leucomalachite green was determined on a Symmetry shieldTMRP18 column with acetonitrile-water as mobile phase, and monitored with fluorescence detector (λem=265 nm andλem=360 nm). The linear range for leucomalachite green is 1～160 ng/ml. The detection limit of the method according to the total weight of malachite green is 1.0 μg/kg. The recovery is in the range of 82.6%～92.6%. |
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| Keywords: | high performance liquid chromatography fluorescence aquatic products malachite green |
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