| 法医DNA提取纯化试剂盒在Hamilton Microlab Starlet DNA自动提取仪上的应用研究 |
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| 引用本文： | 孙敬,赵兴春,姜伯玮,欧元,张建,叶健. 法医DNA提取纯化试剂盒在Hamilton Microlab Starlet DNA自动提取仪上的应用研究[J]. 生命科学仪器, 2010, 0(4): 54-56 |
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| 作者姓名： | 孙敬 赵兴春 姜伯玮 欧元 张建 叶健 |
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| 作者单位： | 公安部物证鉴定中心,北京100038 |
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| 基金项目： | 国家“十一五”科技支撑计划项目课题“法医学DNA提取、纯化试剂及DNA提取仪的研制”（课题编号：2007BAK26B03） |
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| 摘 要： | 本文利用自主研制的法医DNA提取纯化试剂盒在Hamilton Microlab~ Starlet DNA自动提取仪上提取276份血斑样本DNA,PCR扩增检测到270份样本STR分型,成功率达97.8%。结果表明法医DNA提取纯化试剂盒可与国外进口的DNA自动提取仪相配伍进行血斑样本的批量提取。
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| 关 键 词： | 法医DNA提取纯化试剂盒 DNA自动提取仪 血斑样本 |
The application study of forensic DNA extraction and purification kit on Hamilton Microlab Starlet DNA automation workstation |
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| Jing Sun,Xing-chun Zhao,Bo-wei Jiang,Yuan Ou,Jian Zhang,Jian Ye. The application study of forensic DNA extraction and purification kit on Hamilton Microlab Starlet DNA automation workstation[J]. Life Science Instruments, 2010, 0(4): 54-56 |
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| Authors: | Jing Sun Xing-chun Zhao Bo-wei Jiang Yuan Ou Jian Zhang Jian Ye |
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| Affiliation: | (Institute of Forensic Science,Ministry of Public Security P.R.C.,Beijing,100038) |
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| Abstract: | In the study,forensic DNA extraction and purification kit was used to extract a total of 276 blood sample DNA from DNA database on Hamilton Microlab~ Starlet DNA automation workstation.All sample DNA was amplified by PCR.STR genotyping for 270 samples was detected,and the success rate was up to 97.8%.The results indicated that forensic DNA extraction and purification kit can be applied to accomplish lots of sample DNA extraction in coordination with foreign DNA automation workstation. |
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| Keywords: | forensic DNA extraction and purification kit DNA automation workstation blood sample |
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