Radiosensitivity of hepatoma cell lines and human normal liver cell lines exposed in vitro to carbon ions and argon ions at the HIRFL |
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Authors: | Xigang Jing Wenjian Li Wei Wei Dong Lu Jianshe Yang |
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Affiliation: | a Institute Modern Physics, Chinese Academy of Sciences, Gansu Lanzhou, China b Graduate School of Chinese Academy of Sciences, Beijing, China c Life Science School of Northwest Normal University, Gansu Lanzhou, China |
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Abstract: | Human hepatoma (SMMC-7721) and normal liver (L02) cells were irradiated with γ-rays, 12C6+ and 36Ar18+ ion beams at the Heavy Ion Research Facility in Lanzhou (HIRFL). By using the Calyculin-A induced premature chromosome condensation technique, chromatid-type breaks and isochromatid-type breaks were scored separately. Tumor cells irradiated with heavy ions produced a majority of isochromatid break, while chromatid breaks were dominant when cells were exposed to γ-rays. The relative biological effectiveness (RBE) for irradiation-induced chromatid breaks were 3.6 for L02 and 3.5 for SMMC-7721 cell lines at the LET peak of 96 keVμm−112C6+ ions, and 2.9 for both of the two cell lines of 512 keVμm−136Ar18+ ions. It suggested that the RBE of isochromatid-type breaks was pretty high when high-LET radiations were induced. Thus we concluded that the high production of isochromatid-type breaks, induced by the densely ionizing track structure, could be regarded as a signature of high-LET radiation exposure. |
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Keywords: | 87.50.&minus a 87.53.&minus j |
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