|
|||||
|
|
| 食品中正二氢愈疮酸含量的高效液相色谱荧光法测定 | |
| 引用本文： | 段沅杏,贺兵,李干鹏,张承明. 食品中正二氢愈疮酸含量的高效液相色谱荧光法测定[J]. 云南化工, 2011, 38(1): 42-47 |
| 作者姓名： | 段沅杏 贺兵 李干鹏 张承明 |
| 作者单位： | 1. 云南民族大学化学与生物技术学院,云南,昆明,650031;云南省烟草化学重点实验室,云南烟草科学研究院,云南,昆明,650106 2. 云南省烟草化学重点实验室,云南烟草科学研究院,云南,昆明,650106 3. 云南民族大学化学与生物技术学院,云南,昆明,650031 |
| 摘 要： | 建立了高效液相色谱荧光法(HPLC-FLD)测定食品中正二氢愈疮酸含量的方法。采用Luna C18(4.6×150 nm,3.0μm)的色谱柱,以0.1%甲酸乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速1.0 mL/min;激发波长为280nm,发射波长为306nm。在优化的前处理和色谱条件下,正二氢愈疮酸能够与样品基质完全分离,且加标回收率范围为92.43%～99.23%。 |
| 关 键 词： | 正二氢愈疮酸 高效液相色谱法 荧光检测器 |
Simple and Rapid Analysis of Nordihydroguaiaretic Acid Contained in Food by HPLC Fluorescence Detection |
|
| DUAN Chen-xing,HE Bing,LI Gan-peng,ZHANG Cheng-Ming. Simple and Rapid Analysis of Nordihydroguaiaretic Acid Contained in Food by HPLC Fluorescence Detection[J]. Yunnan Chemical Technology, 2011, 38(1): 42-47 | |
| Authors: | DUAN Chen-xing HE Bing LI Gan-peng ZHANG Cheng-Ming |
| Affiliation: | DUAN Yuan-xing1,2,HE Bing2,LI Gan-peng1,ZHANG Cheng-Ming2 (1.Department of Chemistry and Biotechnology,Yunnan Nationalities University,Kunming 650031,China,2.Key Lab of Tobacco Chemistry of Yunnan,Yunnan Academy of Tobacco Science,Kunming 650106,China) |
| Abstract: | Simple and rapid analysis of nordihydroguaiaretic acid（NDGA） in food by HPLC-FLD was developed.The important factors affecting separation and detection were investigated in detail.Extraction of NDGA from samples was accomplished by 90% methanol with 0.1% ascorbic acid.Furthermore,nordihydroguaiaretic acid was separated on a Luna stable bound（4.6×150nm,3.0μm） C18 column with mixture of acetonitrile-water-formic acid（v/v） as the mobile phase and fluorescence detector was effective in isolating NDGA from most of food additives.The standard recoveries were ranged from 93.74%~99.61%.Our results suggest that this is a simply,quickly and sensitively analytical method to detect NDGA in food. |
| Keywords: | Nordihydroguaiaretic acid(NDGA) HPLC FLD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |