| 超声辐照对HepG2细胞存活率影响的实验研究 |
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| 引用本文： | 何年安,王文平,黄备建,朱涛,瞿涤. 超声辐照对HepG2细胞存活率影响的实验研究[J]. 声学技术, 2011, 30(2): 158-160 |
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| 作者姓名： | 何年安 王文平 黄备建 朱涛 瞿涤 |
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| 作者单位： | 1. 复旦大学附属中山医院超声科,上海,200032
2. 复旦大学上海医学院,教育部/卫生部医学分子病毒实验室,上海,200032 |
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| 基金项目： | 上海市重点学科建设资助项目 |
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| 摘 要： | 为探讨超声辐照对体外培养的HepG<,2>细胞存活力的影响,采用正交设计方法,选定声强、占空比和辐照时间3因素,每因素5水平的参数条件设计正交表,对贴壁培养的HepG<,2>细胞进行超声辐照,之后继续培养24h后行MTT(噻唑蓝)实验检测细胞存活率,期间光学显微镜观察培养板内的细胞生长状况及形态.结果表明:当超声声强大...
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| 关 键 词： | 超声 细胞存活率 HepG2细胞 正交设计 |
| 收稿时间： | 2010-06-15 |
| 修稿时间： | 2010-09-14 |
Effect of ultrasound irradiation on viability of HepG2 cell:an experimental study in vitro |
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| HE Nian-an,WANG Wen-ping,HUANG Bei-jian,ZHU Tao and QU Di. Effect of ultrasound irradiation on viability of HepG2 cell:an experimental study in vitro[J]. Technical Acoustics, 2011, 30(2): 158-160 |
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| Authors: | HE Nian-an WANG Wen-ping HUANG Bei-jian ZHU Tao QU Di |
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| Affiliation: | HE Nian-an1,WANG Wen-ping1,HUANG Bei-jian1,ZHU Tao2,QU Di2(1.Department of Ultrasound,Zhongshan Hospital of Fudan University,Shanghai 200032,China,2.Key Laboratory of Medical Molecular Virology,Shanghai Medical College of Fudan University,China) |
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| Abstract: | To investigate the effect of ultrasound irradiation on viability of HepG2 cells in vitro,the parameters of acoustic intensity,duty factor and duration of therapeutic ultrasound are selected,and the ultrasound irradiations are performed according to the orthogonal design.After 24 h of ultrasound irradiations,MTT assays are performed to assess the viability of HepG2 cells.The morphologic change of cells are observed using optical micrography before and after ultrasound irradiation.The results show that,when t... |
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| Keywords: | ultrasound Cell viability HepG2 cell orthogonal design |
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