| 应用流式细胞术检测大鼠心肌细胞缺氧/复氧后内质网应急引起细胞凋亡 |
| |
| 引用本文： | 王珊,廖杰,于力方,李宁,段秋华.应用流式细胞术检测大鼠心肌细胞缺氧/复氧后内质网应急引起细胞凋亡[J].现代仪器使用与维修,2012(5):75-77. |
| |
| 作者姓名： | 王珊 廖杰 于力方 李宁 段秋华 |
| |
| 作者单位： | 解放军总医院医学实验测试中心,北京100853 |
| |
| 摘 要： | 利用流式细胞仪观察西洋参茎叶总皂甘通诱导大鼠心肌细胞后内质网应急引起细胞凋亡。选用原代培养心肌细胞,将心肌细胞分为正常细胞对照组,缺氧/复氧组和缺氧/复氧后加入西洋参茎叶总皂苷组。按照40mg/L,80mg/L,160mg/L浓度培养24h后,应用流式细胞仪采用Annexin-V/PI诱导凋亡。结果显示应用流式细胞仪检测最终浓度为160mg/L西洋参茎叶总皂苷凋亡率明显低于缺氧/复氧组。流式细胞仪检测由内质网应急诱导细胞凋亡对研究细胞凋亡的机理提供一种快速、准确检测方法。
|
| 关 键 词： | 流式细胞术 缺氧/复氧 内质网应急 |
Evaluation of rat cardiomyocyte apoptosis induced by hypoxia/reoxygenation leading to endoplasmic reticulum stress of flow cytometry |
| |
| Wang Shan,Liao Jie,Yu Lifang,Li Ning,Duan Qiuhua.Evaluation of rat cardiomyocyte apoptosis induced by hypoxia/reoxygenation leading to endoplasmic reticulum stress of flow cytometry[J].Modern Instruments,2012(5):75-77. |
| |
| Authors: | Wang Shan Liao Jie Yu Lifang Li Ning Duan Qiuhua |
| |
| Affiliation: | (Medical test center of PLA General hospital, Beijing 100853) |
| |
| Abstract: | The observation of rat cardiomyocyte apoptosis induced by hypoxia/reoxygenation through using flow cytometry.Using original cultured cardiomyocyte,divided into control group,hypoxia/reoxygenation group and Panax quinquefolium saponins（PQS） group.After 24h culturing by different concentration of PQS（40g/L,80g/L,160g/L）,use Annexin-V/PI double staining assay to test apoptosis.The result showed that the apoptosis rate of using concentration of 160g/L PQS group is lower than that of hypoxia/reoxygenation group.Therefore flow cytometry provides a fast,accurate detection method in studying the mechanism of cell apoptosis. |
| |
| Keywords: | Flow cytometry Cell apoptosis Endoplasmic reticulum stress |
| 本文献已被 维普 等数据库收录！ |
|
