| 液相色谱-原子荧光联用测定植物中汞形态 |
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| 引用本文： | 蒲丽梅,曹小丹. 液相色谱-原子荧光联用测定植物中汞形态[J]. 现代仪器使用与维修, 2012, 0(5): 64-67 |
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| 作者姓名： | 蒲丽梅 曹小丹 |
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| 作者单位： | [1]清华大学环境学院环境模拟与污染控制国家重点联合实验室,北京100084 [2]北京吉天仪器有限公司,北京100015 |
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| 基金项目： | 环境模拟与污染控制国家重点联合实验室专项经费 |
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| 摘 要： | 建立液相色谱-原子荧光联用测定植物中汞形态的测量方法,并优化仪器参数和氢化物发生条件。在2%KBH4和7%盐酸的氢化物发生条件下,且Hg^2＋、甲基汞、乙基汞3种汞形态的线性范围在5-100μg/L时,线性相关系数均优于0.999。方法检出限分别是：Hg^2＋为0.33μg/L,甲基汞为0.83μg/L,乙基汞为0.28μg/L,各形态RSD均小于7%,植物样品加标回收率78.7%-112%。本方法适用于植物中汞化合物形态分析测定。
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| 关 键 词： | 液相色谱-原子荧光联用 汞 形态分析 植物 |
Measuring mercury specie determination in plants by liquid chromatoghraphy combined with hydride generation-atomic fluorescence spectrometry |
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| Pu Limei,Cao Xiaodan. Measuring mercury specie determination in plants by liquid chromatoghraphy combined with hydride generation-atomic fluorescence spectrometry[J]. Modern Instruments, 2012, 0(5): 64-67 |
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| Authors: | Pu Limei Cao Xiaodan |
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| Affiliation: | (l.School of environment,Tsinghua Uni-versity State Key Joint Laboratory of Environment Simulation and Pollution Control (SKLESPC), Beijing 100084 2.Beijing Titan Instruments Co. Ltd., Beijing 100015) |
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| Abstract: | Established a method to measure mercury specie determination in plants by using liquid chromatography combined with hydride generation-atomic fluorescence spectrometry（LC-AFS）.The separation condition was optimized.Using 2% KBH4 and 7% HCl,the hydride generation reaction had linear range of 5-100 μg/L for three mercury species and the linear correlation coefficients were greater than 0.999.The method’s detection limits were 0.33μg/L for Hg^2＋,0.38μg/L for methylmercury,0.28μg/L for ethylmercury.RSDs are less than 7% and spiked recoveries are in the range of 78.7%~112%.The proposed method had been applied to the determination of mercury speciation in contaminated plants. |
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| Keywords: | Liquid chromatography combined with atomic fluorescence spectrometry Mercury Speciation analysis Plants |
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