Gas chromatography-mass spectrometry methods for structural analysis of fatty acids |
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Authors: | William W Christie |
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Affiliation: | (1) Scottish Crop Research Institute, Invergowrie, DD2 5DA Dundee, Scotland |
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Abstract: | Procedures for structural analysis of fatty acids are reviewed. The emphasis is on methods that involve gas chromatography-mass
spectrometry and, in particular, the use of picolinyl ester and dimethyloxazoline derivatives. These should be considered
as complementing each other, not simply as alternatives. However, additional derivatization procedures can be of value, including
hydrogenation and deuteration, and preparation of dimethyl disulfide and 4-methyl-1,2,4-triazoline-3,5-dione adducts. Sometimes
complex mixtures must be separated into simpler fractions prior to analysis by gas chromatography-mass spectrometry. Silver
ion and reversed-phase high-performance liquid chromatography are then of special value. In particular, a novel application
of the latter technique, involving a base-deactivated stationary phase and acetonitrile as mobile phase, is described that
is suited to the separation of fatty acids in the form of picolinyl ester and dimethyloxazoline derivatives, as well as methyl
esters. |
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