| Abstract: | HPLC Separation of Tocopherols and Tocotrienols The four tocopherols (T) and four tocotrienols (T3) as well as plastochromanol-8 (P-8) and BHA were separated completely by eluting them from a HPLC diol-column with n-hexane/methyl tert-butylether (96 : 4, v/v). Even for the β- and γ-tocopherol and -tocotrienol isomers, baseline separation was achieved. The method was verified. The method was verified with success on several plant seed oils which were injected directly without any clean-up. For cereals a simplified extraction method was developed and the T, T3 and P-8 content of 9 cereal crops was determined. The separation system showed a long-term stability over six month. |
