| Abstract: | The raw commercial rapeseed lecithin free of erucic acid and glucosinolate (00-type) was purified by deoiling with acetone and extracting with ethanol. The increasing of phosphatidylcholine (PC) content (from 50 to 70–80%) in obtained rapeseed lecithin extract was performed. It was done by column chromatographic fractionation using a low silica gel–lecithin extract ratio about 2:1 and the various temperatures up to 60°C. The fractions were eluted with 95% ethanol. Effect of rapeseed lecithin column fractionation temperatures is significant and the better purification quality in the higher temperatures was observed. Based on performed investigations it could be summarized that the low silica gel–lecithin extract ratio (2:1) allows to obtain rapeseed lecithin with above 80% content of PC and the best results for temperature 60°C were observed. |
