Effect of various concentrations of lead nitrate on the induction of micronuclei in mouse bone marrow

The frequency of micronuclei was evaluated in the bone marrow of mice of either sex administered with 0, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 mg/kg b.wt of lead nitrate at 12, 24 and 36 h post-treatment. The frequency of micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE) increased significantly at 12, 24 and 36 h after treatment with lead nitrate compared to non-drug treated controls. The frequency of micronuclei did not show a dose related increase and the elevation in the frequency of micronuclei was fluctuating type. One important observation which emerged from this study was that the male mice were more sensitive to the induction of micronuclei compared to female mice. This was evidenced by higher frequencies of MPCE in males than females at all the doses for all the post-treatment time periods. The lead nitrate treatment resulted in a spurt in the erythropoiesis as is evidenced by a significant increase in the ratios of polychromatic to normochromatic erythrocytes (P/N ratio) compared to non-drug treated controls at 12, 24 and 36 h post-treatment. The P/N ratio was significantly higher in females than males at 12 and 24 h post-treatment.