碱预处理稻草的酶解糖化

在常温（28℃）下用稀硫酸、氢氧化钠、氨水、过氧化氢溶液处理稻草原料，以实际糖化率为衡量指标对预处理效果进行比较。结果表明2％氢氧化钠溶液预处理效果最好。稻草原料通过温和的碱预处理后，绝大部分木质素被去除，但仍然有超过一半的半纤维素残留。以康宁木霉（T.koningii）QF—02生产的复合酶比两种商品纤维素酶制剂能更有效酶解糖化破预处理稻草。自制复合酶的最适温度和最适pH分别为50℃和4．8；考虑酶解效率和操作费用，酶解时间48h、酶载量10FPU／g稻草、底物浓度8％（w／v）是合理的选择。

Enzymatic Saccharification of Alkali Pretreated Rice Straw

Dilute solutions of H2SO4, NaOH, ammonia and H2o2 were evaluated as pretreatment reagents for improving the enzymatic saccharification of rice straw at room temperature （28 ℃）. The highest saccharification rate was achieved in the case of rice straw pretreated with 2 % NaOH. Mild alkali pretreatment resulted in a majority removal of lignin, but higher than half of hemieellulose was remained in the material. The compound enzyme produced by T. koningii QF-02 was more suitable for the hydrolysis of pretreated flee straw than the mixture of two commercial cellulases. The optimum temperature and pH for the hydrolysis of pretreated rice straw of the compound enzyme was 50℃ and 4.8 respectively. For the hydrolysis of alkali pretreated rice straw by the compound enzyme, the optimal hydrolysis time, enzyme loading and substrate concenlration were determined as 48 h, 10 FPU/g biomass and 8 % （w/v）, respectively.