艾叶提取物的功效性能

目的:明确艾叶提取物作为植物提取物防腐剂的可能性，为开发天然无刺激的植物提取物防腐剂提供参考。方法:通过制备艾叶水及乙醇提取物，测定其主要活性成分含量，分析其对大肠杆菌和金黄色葡萄球菌的抑制效果及稳定性，并探讨其抗氧化活性、紫外吸收性和使用安全性。结果:艾叶乙醇提取物对两种细菌的抑制效果显著，与活性成分的含量存在一定程度的关联性。温度和紫外照射对抑菌活性无明显影响，pH对抑菌活性影响较大。艾叶乙醇提取物在质量浓度为0.96 mg/mL时，对DPPH自由基和ABTS自由基清除率达98%,对超氧阴离子自由基、羟自由基清除率超过50%。质量浓度为0.50 mg/mL时，两种提取物均具有较好的紫外吸收性。鸡胚尿囊膜刺激性试验表明，艾叶提取物在质量浓度为62.50 mg/mL以下时具有较高的安全性。结论:艾叶乙醇提取物具有较好且较为稳定的抑菌效果，以及一定的抗氧化活性、紫外吸收性能和安全性。

Study on efficacy and performance of Artemisia argyi extracts

Objective: By analyzing bacteriostasis and efficacy, the possibility of Artemisia argyi extract to be a plant extract preservative is clarified. It provides a message for the development of natural non-irritating plant extract preservative. Methods: Water and ethanol extracts of A. argyi were prepared. The content of its main active components was determined. Its inhibitory effect and stability on Escherichia coli and Staphylococcus aureus were analyzed. Antioxidant activity, ultraviolet absorption capacity and security were also discussed. Results: The ethanol extract of A. argyi had a significant inhibitory effect on the two kinds of bacteria, which was related to the content of active components to some extent. Temperature and UV irradiation had no significant effect on antibacterial activity, while pH had a greater effect on antibacterial activity. At the concentration of 0.96 mg/mL, ethanol extract of A. argyi leaves could eliminate 98% of DPPH and ABTS free radicals, and more than 50% of superoxide anion and hydroxyl free radicals. When the concentration was 0.5 mg/mL, the two extracts had good ultraviolet absorption capacity. The HET-CAM test showed that the extract of A. argyi had high security at the concentration of 62.5 mg/mL. Conclusion: The ethanol extract of A. argyi has good and stable bacteriostatic effect, certain antioxidant activity, ultraviolet absorption performance and security.