高效液相色谱法测定烟草及烟草制品中的三种水溶性糖

建立了高效液相色谱(HPLC)/示差折光检测器(RID)测定烟草和烟草制品中葡萄糖、果糖和蔗糖的方法。样品用0.01 mol/L 氢氧化钠溶液振荡提取60 min,以高度交联的铅型磺化苯乙烯-二乙烯苯(SDVB)树脂为填料的阳离子交换色谱柱为分离柱,用纯水作流动相进行等梯度洗脱,外标法定量。方法线性良好,相关系数均大于0.999。方法回收率98.9%～101.8%,精密度0.44%～0.90%。方法测定值与离子色谱法的检定结果一致。方法适用于各种烟草和烟草制品中葡萄糖、果糖和蔗糖的分析测定。

Determination of Major Soluble Sugars in Tobacco and Tobacco Products by High Performance Liquid Chromatography

The method of determination of glucose,fructose and sucrose in tobacco and tobacco products was developed by high performance liquid chromatography with a refractive index detector.Tobacco samples were extracted for 60 min by shaking with 0.01 mol/L sodium hydroxide solution.The chromatographic separation was conducted on a cation-exchange column packed with highly cross-linked sulfonated styrenedivinylbenzene resin in the Pb2+ ionic form.The eluent was pure water in isocratic gradient.The external standard method was applied in quantitation.The linearity of the method was good with correlation coefficients above 0.999 in all cases.The recoveries were between 98.9 %-101.8 % and the precision was between 0.44 %-0.90 %.The results determined by the developed method were consistent with those determined by the ion chromatography method.The method is applicable to the determination of fructose,glucose and sucrose in various tobacco and tobacco product samples.