| 绒毛状烟草BAC文库的构建 |
| |
| 引用本文： | 高晓明,陈艳玲,刘贯山,李凤霞,王卫峰,任媛媛,孙玉合.绒毛状烟草BAC文库的构建[J].中国烟草科学,2012,33(3):68-71. |
| |
| 作者姓名： | 高晓明 陈艳玲 刘贯山 李凤霞 王卫峰 任媛媛 孙玉合 |
| |
| 作者单位： | 1. 中国农业科学院烟草研究所,农业部烟草生物学与加工重点实验室,青岛 266101;
2. 华大基因研究院,深圳 518083 |
| |
| 基金项目： | 国家烟草专卖局重点科技项目“烟草cDNA文库构建及重要基因表达谱研究”(110200701021) |
| |
| 摘 要： | 绒毛状烟草是重要的野生种烟草之一。本研究以CopyControlTM pCC1BACTM(Hind Ⅲ Cloning-Ready)Vector为载体,构建了绒毛状烟草的BAC文库。分析结果表明,BAC文库DNA插入片段为50~200 kb,平均110 kb,空载率<2%。随机挑选15个克隆进行稳定性继代试验,100代后插入片段仍然稳定存在。该绒毛状烟草BAC文库的建成,为进一步克隆重要功能基因提供了必备的物质平台。
|
| 关 键 词： | 绒毛状烟草 BAC文库 烟草 |
| 收稿时间： | 2010-07-20 |
BAC Library Construction of Villi Tobacco(Nicotiana tomentosiformis) |
| |
| GAO Xiaoming,CHEN Yanling,LIU Guanshan,LI Fengxia,WANG Weifeng,REN Yuanyuan,SUN Yuhe.BAC Library Construction of Villi Tobacco(Nicotiana tomentosiformis)[J].Chinese Tobacco Science,2012,33(3):68-71. |
| |
| Authors: | GAO Xiaoming CHEN Yanling LIU Guanshan LI Fengxia WANG Weifeng REN Yuanyuan SUN Yuhe |
| |
| Affiliation: | 1. Tobacco Research Institute of CAAS, Key Laboratory of Toabcco Biology and Processing, Ministry of Agriculture, Qingdao 266101, China;
2. Huada Gene Research Institute, Shenzhen 518083, China |
| |
| Abstract: | Villi tobacco(Nicotiana tomentosiformis) is an important species of wild tobacco.The BAC library was constructed with CopyControlTM pCC1BACTM(HindⅢ Cloning-Ready) Vector.From the analysis of randomly selected BAC clones,the inserted DNA size of BAC library ranged from 50 to 200 kb;the average estimated was 110 kb;the empty clone was less than 2%;the insert was stable in a hundred generations. |
| |
| Keywords: | villi tobacco BAC library tobacco |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国烟草科学》浏览原始摘要信息 |
|
点击此处可从《中国烟草科学》下载全文 |
|
