离子色谱-积分脉冲安培法测定烟草中18种游离氨基酸

采用离子色谱-积分脉冲安培法同时测定了烟草中18种游离氨基酸的含量.结果表明,该方法同时检测烟草中18种游离氨基酸的平均回收率为80%～115%,最低检出限为0.03～0.75 μg/mL,线性相关系数为0.9900～0.9992.该方法成功对比分析测定了烤烟、白肋烟和香料烟3种不同类型单料烟以及国内外不同风格卷烟中18种游离氨基酸的含量.

Determination of 18 Free Amino Acids in Tobacco with High Performance Anion-exchange Chromatography with Integrated Pulsed Amperometric Method

18 of free amino acids in tobacco were simultaneously determined by high performance anion-exchange chromatography with integrated pulsed amperometric detection method.The results showed that the recoveries for 18 amino acids were in the range of 80%-115%,the detection limits for 18 amino acids were 0.03-0.75 μg/mL and the calibration graphs of peak area for the analyses were linear over about three orders of magnitude with a correlation coefficient of 0.9900-0.9992.The method was applied to determine 18 free amino acids in tobacco with satisfactory results.The method was employed to determine 18 free amino acids in flue-cured tobacco,burley tobacco and oriental tobacco.The method was also employed to determine successfully 18 free amino acids of different brands of domestic cigarettes and overseas cigarettes.