| 辣根过氧化物酶/聚乙烯醇缩丁醛/碳纳米管修饰玻碳电极检测过氧化氢 |
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| 引用本文： | 韩莉,陶菡,张义明.辣根过氧化物酶/聚乙烯醇缩丁醛/碳纳米管修饰玻碳电极检测过氧化氢[J].中国食品添加剂,2010(2). |
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| 作者姓名： | 韩莉 陶菡 张义明 |
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| 作者单位： | |
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| 基金项目： | 贵州省科学技术基金项目(黔科合J字[2008]2093号) |
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| 摘 要： | 制备了辣根过氧化物酶/聚乙烯醇缩丁醛/碳纳米管修饰玻碳电极的过氧化氢生物传感器。以对苯二酚为电子媒介,采用循环伏安法和电流时间法考察了该传感器对H2O2的催化性能。讨论了媒介体浓度、工作电位、温度、pH对电极响应的影响。结果表明传感器对H2O2表现出良好的电催化性能。在pH=7.0,对苯二酚浓度4.2mmol/L,工作电位-250mV的实验条件下,H2O2浓度在1.67×10-7～1.29×10-5mol/L及1.58×10-5～1.17×10-3mol/L范围内与传感器的电流响应呈线性关系,检出限(S/N=3)为5.554×10-8mol/L。该传感器制备方法简单,成本低,稳定性好,对HO有快速灵敏的响应。
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| 关 键 词： | 辣根过氧化物酶 碳纳米管 聚乙烯醇缩丁醛 过氧化氢 |
Carbon nanotube/polyvinyl butyral/horseradish peroxidase modified glassy carbon electrode for determination of hydrogen peroxide |
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| HAN Li,TAO Han,ZHANG Yi-ming.Carbon nanotube/polyvinyl butyral/horseradish peroxidase modified glassy carbon electrode for determination of hydrogen peroxide[J].China Food Additives,2010(2). |
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| Authors: | HAN Li TAO Han ZHANG Yi-ming |
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| Affiliation: | HAN Li1,TAO Han1,ZHANG Yi-ming1,2(1.Province Key Laboratory of Fermentation Engineering , Biopharmaceutical,Guizhou University,Guiyang 550003,2.Guizhou Industry Polytechnic College,Guiyang 550008) |
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| Abstract: | A hydrogen peroxide biosensor has been made by Horseradish Peroxidase(HRP)-polyvinyl butyral(PVB)-multi-wall carbon nanotube(MWNT)immobilizing on the glassy carbon electrode surface.Using hydroquinone as mediator,the electrocatalytic properties of this sensor toward Hydrogen Peroxide were observed by cyclic voltammetry method and i-t curves.The effect of working potential,pH,temperature and concentration of hydroquinone were discussed in detail.The experimental results indicated that the sensor possessed hi... |
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| Keywords: | horseradish peroxidase carbon nanotube polyvinyl butyral hydrogen peroxide |
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