生物反应器大规模培养脊髓灰质炎病毒

目的应用生物反应器大规模培养脊髓灰质炎病毒。方法用7L、75L和550L生物反应器逐级放大培养Vero细胞,每天取样进行细胞计数。在550L生物反应器中培养脊髓灰质炎病毒,观察细胞病变,并检测病毒感染性滴度。结果一级细胞培养(灌流培养法)、二级细胞培养(循环培养法)和三级细胞培养(批培养法)的平均细胞密度分别为2.79×106、2.38×106和1.04×106个/ml,一级培养的细胞倍增数最高;病毒培养体积达350L,病毒收获液滴度大于7.625lgCCID50∕ml。结论通过三级放大工艺,可大规模培养脊髓灰质炎病毒。

Large-scale Culture of Poliomyelitis Virus in Bioreactor

Objective To culture poliomyelitis virus in a large scale in bioreactor.Methods Vero cells were cultured in 7 L bioreactor,which was scaled up to 75 and 550 L bioreactors,and counted daily.Poliomyelitis virus was inoculated into Vero cells cul-tured in 550 L bioreactor,then the CPE was observed and the infectious titer was determined.Results The densities of Vero cells cultured in 7 L(perfused culture),75 L(circulating culture)and 550 L(batch culture)bioreactors were 2.79×106,2.38×106 and 1.04×106 cells/ml respectively.The multiplication number of Vero cells in 7 L bioreactor was higher than those in 75 and 550 L bioreactors.In 550 L bioreactor,the volume of virus for culture was 350 L,and the titer of harvested virus liquid was more than 7.625 lgCCID50/ml.Conclusion The scaled procedure was suitable for the large-scale culture of poliomyelitis virus.