| RP-HPLC法测定紫苏子、白苏子中乌索酸和齐墩果酸含量 |
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| 引用本文： | 邹盛勤,陈武. RP-HPLC法测定紫苏子、白苏子中乌索酸和齐墩果酸含量[J]. 粮食与油脂, 2008, 0(2): 43-45 |
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| 作者姓名： | 邹盛勤 陈武 |
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| 作者单位： | 宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西宜春,336000 |
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| 基金项目： | 国家高技术研究发展计划(863计划)重点项目 |
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| 摘 要： | 采用反相高效液相色谱法测定紫苏子和白苏子中乌索酸和齐墩果酸含量,色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),流动相为甲醇—水(87:13),光电二极管阵列检测器,检测波长210 nm,流速0.8 ml/min,柱温28℃。乌索酸进样量在0.10～4.00μg,齐墩果酸进样量在0.05～2.00μg时呈现良好线性关系,平均加样回收率分别为100.6%和98.7%,RSD分别为1.6%和1.2%。方法灵敏、准确,重现性好,可用于乌索酸与齐墩果酸含量测定。
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| 关 键 词： | 紫苏子 白苏子 高效液相色谱 乌索酸 齐墩果酸 |
| 文章编号： | 1008-9578（2008）02-0043-03 |
| 收稿时间： | 2007-11-30 |
| 修稿时间： | 2007-11-30 |
Determination of ursolic acid and oleanolic acid in seeds of perilla frutescens(L.)britton by RP-HPLC |
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| ZOU Sheng-qin,CHEN Wu. Determination of ursolic acid and oleanolic acid in seeds of perilla frutescens(L.)britton by RP-HPLC[J]. Cereals & Oils, 2008, 0(2): 43-45 |
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| Authors: | ZOU Sheng-qin CHEN Wu |
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| Abstract: | The contents of ursolic acid and oleanolic acid in seeds of Perilla frutescens (L.) Britton were determined by RP-HPLC. Ursolic acid and oleanolic acid was separated and analyzed on Kromasil C18 column (250 mm×4.6 mm, 5μm) using methanol-water (87:13)as mobile phase at flow rate of 0.8 mL/min. UV detection was at 210 nm and the column temperature was 28℃. An average recovery of 100.6% was obtained with a RSD of 1.6% in the range of 0.10～4.00μg for ursolic acid, and an average recovery or 98.7% was obtained with a RSD of 1.2% in the range of 0.05～2.00μg for oleanolic acid. The method is sensitive, fast and accurate. It can be used for the determination of ursolic acid and oleanolic acid. |
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| Keywords: | seed ofPerilla frutescens HPLC ursolic acid oleanolic acid |
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