Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation |
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| Authors: | Liangju Kuang Nur P Damayanti Chunhui Jiang Xing Fei Wenjie Liu Naagarajan Narayanan Joseph Irudayaraj Osvaldo Campanella Meng Deng |
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| Affiliation: | 1. Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, 47907;2. Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, Indiana, 47907
Bindley Bioscience Center, Purdue University, West Lafayette, Indiana, 47907 |
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| Abstract: | Cell encapsulation within 3D hydrogels is an attractive approach to develop effective cell-based therapies. However, little is known about how cells respond to the dynamic microenvironment resulting from hydrogel gelation-based cell encapsulation. Here, a tunable biomimetic hydrogel system that possesses alterable gelation kinetics and biologically relevant matrix stiffness is developed to study 3D dynamic cellular responses during encapsulation. Hydrogels are synthesized by crosslinking thiolated hyaluronic acid and thiolated chondroitin sulfate with poly(ethylene glycol) diacrylate under cell-compatible conditions. Hydrogel properties are tailored by altering thiol substitution degrees of glycosaminoglycans or molecular weights of crosslinkers. Encapsulation of human mesenchymal stem cells through hydrogel gelation reveals high cell viability as well as a three-stage gelation-dependent cellular response in real-time focal adhesion kinase (FAK) phosphorylation in live single cells. Furthermore, stiffer hydrogels result in higher equilibrium FAK activity and enhanced actin protrusions. Our results demonstrate the promise of hydrogel-mediated cellular responses during cell encapsulation. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47212. |
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| Keywords: | cell encapsulation focal adhesion kinase glycosaminoglycans human mesenchymal stem cells hydrogels |
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