介质阻挡等离子体降解阿月浑子坚果仁中AFB1的效果研究

探究介质阻挡(Dielectric barrier discharge，DBD)等离子体技术对阿月浑子坚果仁中黄曲霉毒素B1（Aflatoxin B1，AFB1）的降解影响因素。采用高效液相色谱法（High performance liquid chromatography，HPLC）分析AFB1，通过单因素实验，考察放电时间、放电功率和放电间距对AFB1降解率的影响，在此基础上进行Box-Behnken的实验设计，选取AFB1降解率作为响应值，优化AFB1的降解条件。根据AFB1浓度和峰面积建立标准工作曲线，得到曲线方程为y=57773.964 x+166368.663，R2=0.9995。通过响应面优化可知，各因素对AFB1降解率的影响大小依次为放电功率>放电间距>放电时间。DBD等离子体降解AFB1的最佳工艺条件为放电时间20 s、放电功率200 W和放电间距0.4 cm，AFB1的降解率高达93.4287%，与预测值98.5162%基本一致，说明该模型优化较为合理。DBD等离子体对阿月浑子坚果仁中AFB1具有良好的降解效果，DBD等离子体降解真菌毒素有广阔的应用前景。

Effect of dielectric barrier discharge plasma for degradation of AFB1 in pistachios

To investigate the degradation of dielectric barrier discharge(DBD) plasma technology on aflatoxin B1 (AFB1) in the nuts. The degradation effect of high performance liquid chromatography (HPLC) method by dielectric barrier discharge plasma on AFB1 was analyzed. It investigated the effect of discharge time, discharge power and discharge distance on AFB1 degradation rate. On this basis, Box-Behnken experiment was designed, selected AFB1 degradation rate as the response value, and optimized the degradation conditions of AFB1 was performed. The standard working curve of the AFB1 concentration and peak area yields the curve equation of y=57773.964 x + 166368.663 and R2=0.9995. Through the response surface optimization, the influence of each factor on the size of AFB1 degradation rate is successively the discharge power> discharge distance> discharge time.The optimal process conditions for dielectric barrier discharge plasma degradation of AFB1 are the discharge time of 20 s, a discharge power of 200 W, and a discharge distance of 0.4 cm.The degradation rate of AFB1 is as high as 93.4287%, which is basically consistent with the predicted value of 98.5162%, indicating that the model optimization is more reasonable. Dielectric barrier discharge plasma has good degradation effect of AFB1 in the pistachios and has broad application potential as non-thermal processing technology.