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Characterization and in vitro evaluation of electrospun aligned-fiber membranes of poly(L-co-D,L-lactic acid) |
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| Authors: | Claudenete Vieira Leal Rosemeire dos Santos Almeida José Luis Dávila Juliana Almeida Domingues Moema A. Hausen Eliana A. Rezende Duek Marcos Akira d’Ávila |
| Affiliation: | 1. Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, University of Campinas (UNICAMP), Campinas, CEP 13083-860, Brazil;2. Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, University of Campinas (UNICAMP), Campinas, CEP 13083-860, Brazil Faculty of Technology Arthur de Azevedo (FATEC-Mogi Mirim), Mogi Mirim, CEP 13801-005, Brazil Faculty of Technology (FATEC-Mauá), Mauá, CEP 09390-120, Brazil;3. Department of Cellular and Structural Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, CEP 13083-862, Brazil;4. Faculty of Medical Sciences, Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, CEP 18030-095, Brazil;5. Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, University of Campinas (UNICAMP), Campinas, CEP 13083-860, Brazil Faculty of Medical Sciences, Pontifical Catholic University of São Paulo (PUC-SP), Sorocaba, CEP 18030-095, Brazil |
| Abstract: | poly(L-co-D,L-lactic acid) (PLDLA) is a bioresorbable and biocompatible copolymer obtained from the combination of L -lactic and D , L -lactic monomers. PLDLA membranes formed by aligned fibers were prepared by AC/DC electrospinning. Solutions of 8 and 10 wt % of PLDLA were used and membranes were collected by a rotating cylindrical collector. The results showed a slight increase in the fiber diameter with the increase in the polymer concentration. Fourier transform infrared spectroscopy results showed no changes in the chemical structure. Dynamic mechanical analysis results showed an increase in the storage modulus and a decrease in glass-transition temperature for the 10 wt % samples, which was corroborated by differential scanning calorimetry. Thermogravimetric analysis showed a slight variation of the peak degradation temperature. Nevertheless, the samples did not present solvent residues. The membranes were tested in vitro using adipose-derived mesenchymal stem cells (ASCs). The ASCs proliferated after 1, 7, and 14 days of culture, maintaining a spindle-like morphology, which evidences their potential for tissue engineering applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47657. |
| Keywords: | biomaterials electrospinning fibers membranes |