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鸡卵黄抗体(IgY)的诱导
引用本文:范龙彬,杨曜中,俞建瑛,宋聿文,欧伶. 鸡卵黄抗体(IgY)的诱导[J]. 中国生物制品学杂志, 2000, 13(2): 79-81
作者姓名:范龙彬  杨曜中  俞建瑛  宋聿文  欧伶
作者单位:范龙彬(华东理工大学应用生物系,上海 200237)      杨曜中(华东理工大学应用生物系,上海 200237)      俞建瑛(华东理工大学应用生物系,上海 200237)      宋聿文(华东理工大学应用生物系,上海 200237)       欧伶(华东理工大学应用生物系,上海 200237)
摘    要:目的 获得鸡卵黄抗体(Igy),并研究该抗体在鸡的黄液中表达的进程。方法 用吗啡—牛血清蛋白 结合物为模型抗原对30周龄产蛋鸡进行免疫诱导,用ELISA间接法测定不同时间的抗体滴度。结果全程免疫过 程中白蛋白抗体最高滴度为1:12800,吗啡抗体最高滴度为 1:1600,经 5次免疫后(42周龄)抗体滴度不再升高。结论 为使目的抗体在鸡卵黄液中持续而高质量诱导奠定基础。

关 键 词:  卵黄  IgY
修稿时间:1999-01-08

Induction of Egg Yolk Antibody(IgY)
Fan Longbin,Yang Yaozhong,Yu Jianying. Induction of Egg Yolk Antibody(IgY)[J]. Chinese Journal of Bilogicals, 2000, 13(2): 79-81
Authors:Fan Longbin  Yang Yaozhong  Yu Jianying
Affiliation:Fan Longbin,Yang Yaozhong,Yu Jianying(Department of Applied Biology, East China University of Science and Technology, Shanghai 200237)
Abstract:Objective To obtain IgY and study on the expression of it in egg yolk,Methods The laying hens aged 3Ow were immunized with morphine-bovine serum albumin conjugate,and the IgY induced was detected by indirect ELISA.Results The highest biers of antibodies to albumin and morphine were 1: 12800 and 1:1600 respectively. However, no increase of the biers were observed in the hens(aged 42w)after being immunized with 5 doses of the conjugate.Conclusion The study laid a foundation of the induction and persistence of goal antibody in egg yolk.
Keywords:Eggyolk lgY Morphine Bovine serum albumin
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