| HPTLC-DPPH法定性定量分析保健品中芦丁 |
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| 引用本文： | 王天楠,张煌,陈益胜,徐学明,黄彩虹. HPTLC-DPPH法定性定量分析保健品中芦丁[J]. 食品与机械, 2019, 0(1): 92-96 |
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| 作者姓名： | 王天楠 张煌 陈益胜 徐学明 黄彩虹 |
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| 作者单位： | 江南大学食品学院食品科学国家重点实验室;河南牧业经济学院食品与生物工程学院;江南大学食品学院 |
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| 基金项目： | 国家重点研发计划(编号：2017YFF0207800)；江苏省自然科学基金(编号：BK20170177)；中国博士后基金(编号：2017M610298)；江苏省博士后基金(编号：1701021A) |
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| 摘 要： | 将1,1-二苯基-2-三硝基苯肼(DPPH)还原变色反应与高效薄层色谱分离相结合,建立了一种HPTLC-DPPH联用测定保健品中芦丁的方法,并考察了DPPH显色反应时间、流动相配比、光密度扫描参数对色谱分离、扫描效果的影响,同时测试了该方法的回收率和精密度。结果显示,该方法的标准添加回收率为82%～97%,精密度RSD10%,且可消除保健品中复杂基质的干扰,适用于保健品中芦丁的快速测定。
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| 关 键 词： | 芦丁；保健品；HPTLC；DPPH |
| 收稿时间： | 2018-10-22 |
Reliable screening of Rutin in healthcare products by HPTLC-DPPH assay |
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| WANGTiannan,ZHANGHuang,CHENYisheng,XUXueming,HUANGCaihong. Reliable screening of Rutin in healthcare products by HPTLC-DPPH assay[J]. Food and Machinery, 2019, 0(1): 92-96 |
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| Authors: | WANGTiannan ZHANGHuang CHENYisheng XUXueming HUANGCaihong |
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| Affiliation: | State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;Henan University of Animal Husbandry and Economy, Zhengzhou, Henan 400045, China;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China |
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| Abstract: | The study combines the reaction of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) with reduce discoloration and high-performance thin layer chromatography to establish a new method for the determination of rutin in healthcare products by HPTLC-DPPH. The effects of DPPH color reaction time, mobile phase, and optical density scanning parameters on chromatographic separation and densitometric results are investigated as well as recovery and precision of the method. The standard addition recovery rate of the method is 82%~97%, and the precision RSD is <10%. It was demonstrated that the method is able to circumvent the interferences from sample matrices with a simplified sample pretreatment. Therefore, the method is suitable for rapid detection of rutin in healthcare products. |
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| Keywords: | Rutin Healthcare products HPTLC DPPH |
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