云南盐津乌骨鸡与武定鸡肌肉蛋白质组学差异研究

选取云南两种具有代表性的地方优质鸡种盐津乌骨鸡和武定鸡为研究对象,采用双向电泳(2DE)分析其腿肌与胸肌中蛋白表达差异,并以基质辅助激光解析电离-串联飞行时间质谱(MALDI-TOF/TOF MS)鉴定腿肌与胸肌中差异蛋白质点。结果表明,经双向电泳图谱分析所获得的肌肉组织蛋白点分布于pH4.0~7.0之间,其中盐津乌骨鸡腿肌、胸肌中分别检测到(1421±34)、(1329±25)个蛋白点;武定鸡腿肌、胸肌中检测到(1585±19)、(1516±28)个蛋白质点。对比分析盐津乌骨鸡与武定鸡的DE图谱,发现有178个点存在明显的表达差异,在盐津乌骨鸡中高表达的点有72个,在武定鸡中高表达的点有106个。选取的蛋白质点经鉴定主要为核纤层蛋白A、磷酸葡萄糖变位酶1、β-烯醇酶、原肌球蛋白1、三磷酸腺苷合成酶、C-反应蛋白前体、B链α-晶状体蛋白、肌酸激酶M型、乙二醛酶1、磷酸甘油酸激酶 PGK1、慢骨肌肌钙蛋白T、蛋白酶体非ATP酶调节亚单位14、热休克蛋白β-1超氧化物歧化酶、以及一些假设蛋白和非特征性蛋白。

Differental Proteomic Analysis in Breast and Leg Muscles from Yunnan Yanjin Silky Fowl Chicken and Wuding Chicken

In the study,Yanjin silky fowl chicken and Wuding Chicken were chosen to analyze the protein spots expressed differently from the breast muscle and leg muscle by two-dimensional gel electrophoresis( DE). And the peptide mapping fingerprint was obtained by matrix-assisted laser desorption ionization-time of flight-time of flight-mass spectrometry( MALDI-TOF/TOF MS).The results showed that the protein spots were distributed mainly within the range of pH4.0~7.0.For Yanjin silky fowl chicken leg muscle and breast muscle,the average protein spots detected were( 1421 ± 34) and( 1329 ± 25) spots,respectively.For Wuding Chicken leg muscle and breast muscle,the average protein spots detected were( 1585 ± 19) and( 1516± 28) spots,respectively.There were 178 protein spots expressed differently by analyzing the 2-DE maps between YanJin silky fowl chicken muscle and Wuding Chicken muscle.For Yanjin silky fowl chicken muscle,there were 72 protein spots expressed higher,and for Wuding Chicken muscle,106 protein spots expressed higher. The protein spots were identified as lamin-A,phosphoglucomutase 1,beta-enolase,isoform 1 of tropomyosin alpha-1 chain,ATP synthase subunit beta,C-reactive protein precursor,alpha-crystallin B chain,creatine kinase M-type,glyoxylase 1,phosphoglycerate,slow skeletal muscle troponin T,proteasome non-ATPase regulatory subunit 14,heat shock protein beta-1,superoxide dismutase and some hypothetical proteins and uncharacterized proteins.