西双版纳12种食用香料植物精油抑制炎症因子NO活性研究

为了进一步寻找新型高效抗炎药物,本实验选取西双版纳地区12种香料植物,采用水蒸汽蒸馏法提取精油,以脂多糖(Lipopolysaccharides,LPS)刺激RAW264.7细胞,用MTS试剂盒评估细胞活力、Griess试剂盒评估NO产生水平。通过GC-MS联用技术对具有显著抑制作用的精油进行成分分析。结果表明:蒜香藤精油在浓度为0.004%及0.005%时对细胞活力有显著的抑制作用,马来树胡椒精油在浓度为0.005%时,对细胞活力有显著的抑制作用,其他精油在0.001%~0.005%浓度范围内对细胞活力无显著影响或有一定的促进作用;马来树胡椒、锡兰肉桂、清香木、毛叶丁香罗勒、蒜香藤和水香薷具有显著(p<0.0001)的抑制NO产生作用,且其抑制炎症因子NO活性结果由强至弱为:马来树胡椒 > 水香薷 > 锡兰肉桂 > 清香木 > 蒜香藤 > 毛叶丁香罗勒,其中马来树胡椒抑制NO率最强为43.68%,除毛叶丁香罗勒精油外,其余抑制活性均优于阳性对照地塞米松;通过GC-MS对六种精油成分进行分析发现,精油成分以烯烃为主。六种精油抑制炎症因子NO产生作用虽然存在差异,但作为一种新型天然抑制炎症NO的材料在药用及功能食品等方面具有较大的潜力。

Activities of Inhibiting Inflammatory Factor NO of Essential Oils from 12 Edible and Fragrant Plants in Xishuangbanna

To find the new and efficient anti-inflammatory medicine, twelve edible and fragrant plants were collected in Xishuangbanna Tropical Botanical Garden. Essential oils were extracted by steam distillation. For anti-inflammatory activity, the cell viability and nitric oxide (NO) production were determined on lipopolysaccharide (LPS) -stimulated RAW264.7 cells by MTS assay and the Griss reagent system, respectively. For the excellent effects on inflammatory, the compounds of them were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The result showed that Mansoa alliacea inhibited cell viability under 0.004% and 0.005% significantly, Piper arborescens inhibited cell viability under 0.005% slightly. Other essential oils had no effects on cell viability or were beneficial to cell viability. Piper arborescens, Cinnamomum verum, Pistacia weinmannifolia, Ocimum Gratissimum var. suave, Mansoa alliacea, Elsholtzia kachinensis exerted efficient effects on production of NO (p<0.0001), the activity that inhibited production of NO of the six essential oil changed from stronger to weaker as:P. arborescens > E. kachinensis > C. verum > P. weinmannifolia > M. alliacea > O. Gratissimum var. suave. P. arborescens essential oil displayed the strongest inhibiting activity on production of NO which inhibitory rate reached 43.68%. They exerted efficient effects more than Dex except for O. Gratissimum var. suave. It was found that the main components of this six essential oils were alkene by GC-MS. The inhibitory activity of production of NO among these plant essential oils had difference, but these essential oils as a material that new and natural had large potential in medicine and functional foods.