保健食品中人参皂苷类成分的快速检测及应用

目的建立保健食品中人参皂苷类成分的提取、检测方法,并对检测方法进行方法学考察。方法以标示含人参成分保健食品为研究对象,样品经溶剂提取,固相萃取小柱净化,超高效液相-串联二极管阵列检测器检测保健食品中人参皂苷类成分。使用建立的方法对市售17批保健食品(标示成分为总皂苷)中的人参皂苷类成分进行含量测定,并与产品总皂苷标示量和传统检测方法的测定结果进行对比,评判含人参成分保健食品品质。结果建立的方法能够快速、准确、灵敏地检测7种皂苷成分。17批标示含人参成分的保健食品所测人参皂苷类成分总量均低于采用《规范》方法所测得的总皂苷含量和产品总皂苷标示含量。结论本研究建立的方法可以客观反映含人参成分保健食品的品质。

Rapid determination of ginsenosides in health food and its application

Objective To establish a method for extraction and determination of ginsenosides in health food, and investigate the method of detection. Methods Taking health food labeled with Panax ginseng as the research object, the samples were extracted by solvent, purified by solid phase extraction column, and the ginsenosides in health food were detected by ultra-high performance liquid-series diode array detector (UPLC-DAD). The established method was used to determine the content of ginsenosides in 17 batches of health food (labeled as total saponin) on the market, and compared with the results of total saponin labeling and traditional detection methods. The quality of health food containing ginsenosides were evaluated. Results The established method was rapid, accurate and sensitive for determination of 7 kinds of ginsenosides. The total amount of ginsenosides in 17 batches of health food labeled with ginseng ingredients was lower than the total content of ginsenosides and the total content of products labeled with ginsenosides measured by the method of Specification. Conclusion The method established in this study can objectively reflect the quality of health food containing Panax ginseng.